| Autor: |
Willets, K. A., Nishimura, S. Y., Schuck, P. J., Twieg, R. J., Moerner, W. E. |
| Zdroj: |
Accounts of Chemical Research; July 2005, Vol. 38 Issue: 7 p549-556, 8p |
| Abstrakt: |
Fluorescence imaging of single molecules at room temperature is a powerful technique for studying complex condensed phase systems and revealing structure and dynamics hidden by ensemble measurements. Successful single-molecule spectroscopic experiments rely upon strong emitters that can be detected at the level of individual copies above the relevant background signals. This Account discusses a class of nonlinear optical chromophores that not only are well-suited for single-molecule imaging but also offer additional beneficial properties such as a significant ground-state dipole moment, moderate hyperpolarizability, and sensitivity to local environment. An overview of the photophysical properties of several members of this class of molecules as well as a mechanism to help understand the environmental sensitivity is presented. Some preliminary applications of the chromophores as single-molecule reporters in cellular and polymer systems are discussed, along with detection of the emitters by two-photon fluorescence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
