| Autor: |
Luque, E.A., Lemanski, L.F., Dube, D.K. |
| Zdroj: |
Biochemical and Biophysical Research Communications; August 1994, Vol. 203 Issue: 1 p319-325, 7p |
| Abstrakt: |
The cDNA for alpha-tropomyosin (TM) was cloned by the polymerase chain reaction (PCR) from a lambda gt11 library constructed with mRNA from juvenile axolotl heart tissues. Subsequently, the nucleotide sequence of the cDNA was determined. This is the first reported cDNA for axolotl alpha-tropomyosin. Comparative analyses of the deduced amino acid sequence of this cDNA with Xenopusskeletal muscle alpha-tropomyosin sequences indicate that the axolotl heart cDNA has 93% and 96% homology in the regions of amino acids 39-80 (exon 2b) and 258-284 (exon 9a), respectively. However, there is about 86.55% homology at the nucleic acid level (coding region) and 97.5% homology at the amino acid level with that of Xenopusskeletal muscle alpha-tropomyosin cDNA. Northern blot analyses with polyA+ RNA from juvenile heart suggest the presence of at least two different transcripts for alpha-tropomyosin in axolotl heart. The results of 3′-RACE concur with those of northern blot analyses. |
| Databáze: |
Supplemental Index |
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