| Autor: |
Spatz, A.Martin, Ali, Stuart A., Auer, Manfred, Graf, Christine, Eibl, Martha M., Steinkasserer, Alexander |
| Zdroj: |
Biochemical and Biophysical Research Communications; October 1998, Vol. 251 Issue: 1 p235-238, 4p |
| Abstrakt: |
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % α-helix and 17.5 % β-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies. |
| Databáze: |
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