| Autor: |
Heath, I.Brent, Gupta, Gagan, Bai, Suk |
| Zdroj: |
Fungal Genetics and Biology; June 2000, Vol. 30 Issue: 1 p45-62, 18p |
| Abstrakt: |
The organization and roles of F-actin and microtubules in the maintenance and initiation of hyphal tip growth have been analyzed in Saprolegnia feraxand Neurospora crassa.In hyphae of both species, the apex is depleted of microtubules relative to subapical regions and near-normal morphogenesis occurs in concentrations of nocodazole or MBC which remove microtubules, slow growth, and disrupt nuclear positioning. In contrast, each species contains characteristic tip-high arrays of plasma membrane-adjacent F-actin, whose organization is largely unaltered by the loss of microtubules but disruption of which by latrunculin B disrupts tip morphology. Hyphal initiation and subsequent normal morphogenesis from protoplasts of both species and spores of S. feraxare independent of microtubules, but at least in S. feraxobligatorily involve the formation of F-actin caps adjacent to the hyphal tip plasma membrane. These observations indicate an obligatory role for F-actin in hyphal polarization and tip morphogenesis and only an indirect role for microtubules. |
| Databáze: |
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