| Autor: |
Lauter, Frank-Roman, Marchfelder, Uta, Russo, Vincenzo E.A., Yamashiro, Carl T., Yatzkan, Einat, Yarden, Oded |
| Zdroj: |
Fungal Genetics and Biology; April 1998, Vol. 23 Issue: 3 p300-310, 11p |
| Abstrakt: |
Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that inNeurospora crassahyphae branch more frequently in cultures grown in light. Previous studies had identifiedcot-1as a gene that controls apical hyphal cell elongation. In thecot-1mutant, cessation of elongation is accompanied by hyperbranching. Here we demonstrate that thecot-1gene encodes two transcript species of about 2100 nt (cot-1(s)) and about 2400 nt (cot-1(l)) in length and that the ratio of both transcript species abundance is photoregulated. The origin of the difference betweencot-1(l) andcot-1(s) was localized to the 5′ end of thecot-1transcripts, suggesting that two COT1 isoforms with different activities may be formed. Both light effects, on branching and oncot-1expression, were dependent on functionalwc-1andwc-2gene products. In addition to light,l-sorbose comprises another environmental cue that controls hyphal branching inN. crassa.In the presence ofl-sorbose, photoregulation ofcot-1was blocked, suggesting the involvement of alternative and potentially interdependent signaling pathways for the regulation of hyphal elongation/branching. |
| Databáze: |
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