| Autor: |
Yu, Jen Z., Bondy, Gregory P., Allard, Michael F., Thompson, Christopher R., Levin, Adeera, McManus, Bruce M. |
| Zdroj: |
Journal of Molecular and Cellular Cardiology; December, 1996, Vol. 28 Issue: 12 p2429-2441, 13p |
| Abstrakt: |
Left ventricular hypertrophy is very prevalent among patients with renal insufficiency. Known hypertrophic factors, such as systemic hypertension, do not adequately account for the prevalence of left ventricular hypertrophy in these patients. Circulating growth factors may stimulate cardiomyocyte growth and contribute to the development of left ventricular hypertrophy. The effects of sera from patients with (n=30) and without (n=5) chronic renal insufficiency on the growth of cultured adult cardiomyocytes were compared. An adult rat cardiomyocyte primary culture system was established with a high purity of cardiomyocyte population as confirmed by immunocytochemical staining of cardiac contractile proteins. Myocytes responded with increased [3H]thymidine incorporation when treated with angiotensin II, epidermal growth factor, hydrocortisone and insulin, and with increased [3H]phenylalanine incorporation when treated with parathormone, isoproterenol, phenylephrine and insulin. Renal insufficiency serum stimulated [3H]thymidine incorporation was 1.5 times that of the control (P<0.02) and also tended to increase incorporation of [3H]phenylalanine compared to the control (P= |
| Databáze: |
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