| Autor: |
Charles, Hubert, Heddi, Abdelaziz, Guillaud, Josette, Nardon, Christianne, Nardon, Paul |
| Zdroj: |
Biochemical and Biophysical Research Communications; October 1997, Vol. 239 Issue: 3 p769-774, 6p |
| Abstrakt: |
Specific proteins of symbiosis were analyzed by the comparison of two-dimensional electrophoresis protein patterns of symbiotic and aposymbiotic strains of the weevilSitophilus oryzae.One protein was shown to be exclusively expressed in the aposymbiotic strain and three proteins, including a chaperonin, were characterized in the symbiotic strain pattern. ThegroE-like operon, encoding the two chaperonins groES and GroEL-like proteins of the endocytobiotes, was sequenced. It was found to be very similar to thegroEoperon ofEscherichia coli(82% identity).In vitroandex vivoexperiments of protein labelling demonstrated that almost 40% of the endocytobiote protein synthesisex vivois focused on the GroEL-like protein. Finally, we showed by northern blotting that heat shock at 38°C results ingroELmRNA accumulation inside the endocytobiotes. This work supports the hypothesis that chaperonins could have an essential physiological function in the maintenance of the symbiotic association. |
| Databáze: |
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