| Autor: |
Veale, Margaret F., Roberts, Natalie J., King, Glenn F., King, Nicholas J.C. |
| Zdroj: |
Biochemical and Biophysical Research Communications; October 1997, Vol. 239 Issue: 3 p868-874, 7p |
| Abstrakt: |
Mobile lipids detected using1H-NMR in stimulated lymphocytes were correlated with cell cycle phase, expression of the interleukin-2 receptor α and proliferation to assess the activation status of the lymphocytes. Mobile lipid levels, IL-2Rα expression and proliferation increased after treatment with PMA and ionomycin. PMA or ionomycin stimulation alone induced increased IL-2Rα expression but not proliferation. PMA- but not ionomycin-stimulation generated mobile lipid. Treatment with anti-CD3 antibody did not increase IL-2Rα expression or proliferation but did generate increased amounts of mobile lipid. The cell cycle status of thymocytes treated with anti-CD3, PMA or ionomycin alone indicated an accumulation of the cells in the G1phase of the cell cycle. The generation of mobile lipid was abrogated in anti-CD3 antibody-stimulated thymic lymphocytes but not in splenic lymphocytes, using a phosphatidylcholine-specific phospholipase C (PC-PLC) inhibitor which blocked cells in the G1/S phase of the cell cycle. This suggests that the1H-NMR-detectable mobile lipid may be generated in anti-CD3 antibody-stimulated thymic lymphocytes by the action of PC-PLC activity via the catabolism of PC, in the absence of classical signs of activation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect