| Autor: |
Bellaoui, Mohammed, Keddie, James, Gruissem, Wilhelm |
| Zdroj: |
Plant Molecular Biology; November 2003, Vol. 53 Issue: 4 p531-543, 13p |
| Abstrakt: |
The defective chloroplast and leaf-mutable(dcl-m) mutation of tomato blocks chloroplast differentiation in leaf mesophyll cells and a signaling system that appears to be required for morphogenesis of palisade cells during leaf growth. To dissect the function of DCL, mutants with stable dclalleles (dcl-s) were generated and examined for their phenotype. DCL/dcl-splant produce dcl-s/dcl-sseeds with embryos arrested at the globular stage of development. The levels of several chloroplast- and nuclear-encoded proteins are strongly reduced in dcl-mmutant leaf sectors without significant changes in their corresponding mRNAs. The 4.5S rRNA fails to be processed efficiently, however, suggesting that DCL has a direct or indirect function in rRNA processing or correct ribosome assembly. Accordingly, chloroplasts in dcl-msectors are impaired in polysome assembly, which can explain the reduced accumulation of chloroplast-encoded proteins. These results suggest that DCL is required for chloroplast rRNA processing, and emphasize the importance of plastid function during embryogenesis. |
| Databáze: |
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