| Autor: |
Scherrer, Sandra, De Vries, Onno M.H, Dudler, Robert, Wessels, Joseph G.H, Honegger, Rosmarie |
| Zdroj: |
Fungal Genetics and Biology; June 2000, Vol. 30 Issue: 1 p81-93, 13p |
| Abstrakt: |
In the symbiotic phenotype of the lichen-forming ascomycetes Xanthoria parietinaand X. ectaneoides,a conglutinate, hydrophilic cortex surrounds a system of aerial hyphae with hydrophobic wall surfaces. In X. parietinafreeze-fracture electron microscopy showed that a rodlet layer covers the fungal and algal wall surfaces. Extracts of hot SDS-insoluble wall residues isolated from both species contained a protein that revealed a rodlet layer upon interfacial self-assembly. The N-terminal sequence of the 10-kDa protein of X. ectaneoidesserved to clone cDNA fragments of XEH1(H1of X. ectaneoides) and XPH1(H1of X. parietina) by RT–PCR. Genomic DNA blot analysis with both lichenized species and the aposymbiotically cultured symbionts of X. parietinashowed that XPH1and XEH1are fungal single copy genes. The deduced amino acid sequences of the two encoded proteins were 96% identical and showed the characteristics of class I hydrophobins. |
| Databáze: |
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