Crystallization and Preliminary Crystallographic Analysis of Antistasin, a Leech-derived Inhibitor of Blood Coagulation Factor Xa

Autor: Schreuder, Herman, Arkema, Anita, de Boer, Bijtske, Kalk, Kor, Dijkema, Rein, Mulders, John, Theunissen, Henri, Hol, Wim
Zdroj: JMB Online (Journal of Molecular Biology); June 1993, Vol. 231 Issue: 4 p1137-1138, 2p
Abstrakt: The salivary gland of the Mexican leech Haementeria officinaliscontains a 15 kDa protein which is a potent and selective inhibitor of factor Xa. It inhibits not only blood coagulation, but also metastasis. A gene, coding for a sequence similar to published antistasin sequences, has been synthesized and expressed in Chinese hamster ovary (CHO) cells. The recombinant protein was purified and crystallized at pH 6·0, using 31% ammonium sulfate as a precipitant, the crystals diffract at least to 2·8 Å. The spacegroup is I422 with a= b= 77·7 Å and c= 88·4 Å. The crystals contain 42% solvent and one protein molecule in the asymmetric unit. A search for heavy atom derivatives is in progress.
Databáze: Supplemental Index