| Autor: |
Ron, D, Napolitano, E W, Voronova, A, Vasquez, N J, Roberts, D N, Calio, B L, Caothien, R H, Pettiford, S M, Wellik, S, Mandac, J B, Kauvar, L M |
| Zdroj: |
Journal of Biological Chemistry; July 1999, Vol. 274 Issue: 27 p19003-10, 8p |
| Abstrakt: |
The protein kinase C (PKC) family has been clearly implicated in T-cell activation as have several nonreceptor protein-tyrosine kinases associated with the T-cell receptor, including p59fyn. This report demonstrates that thetaPKC and p59fyn specifically interact in vitro, in the yeast two-hybrid system, and in T-cells. Further indications of direct interaction are that p59fyn potentiates thetaPKC catalytic activity and that thetaPKC is a substrate for tyrosine phosphorylation by p59fyn. This interaction may account for the localization of thetaPKC following T-cell activation, pharmacological disruption of which results in specific cell-signaling defects. The demonstration of a physical interaction between a PKC and a protein-tyrosine kinase expands the class of PKC-anchoring proteins (receptors for activated C kinases (RACKs)) and demonstrates a direct connection between these two major T-cell-signaling pathways. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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