| Autor: |
Svetlov, V, Nolan, K, Burgess, R R |
| Zdroj: |
Journal of Biological Chemistry; May 1998, Vol. 273 Issue: 18 p10827-30, 4p |
| Abstrakt: |
Stoichiometry of the third largest subunit (Rpb3) of the yeast RNA polymerase II is a subject of continuing controversy. In this work we utilized immunoaffinity and nickel-chelate chromatographic techniques to isolate the RNA polymerase II species assembled in vivo in the presence of the His6-tagged and untagged Rpb3. The distribution pattern of tagged and untagged subunits among the RNA polymerase II molecules is consistent with a stoichiometry of 1 Rpb3 polypeptide per molecule of RNA polymerase. Deletion of either alpha-homology region (amino acids 29-55 or 226-267) from the Rpb3 sequence abolished its ability to assemble into RNA polymerase II in vivo. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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