A novel serine protease inhibition motif involving a multi-centered short hydrogen bonding network at the active site11Edited by D. Rees

Autor: Katz, Bradley A, Elrod, Kyle, Luong, Christine, Rice, Mark J, Mackman, Richard L, Sprengeler, Paul A, Spencer, Jeffrey, Hataye, Jason, Janc, James, Link, John, Litvak, Joane, Rai, Roopa, Rice, Ken, Sideris, Steve, Verner, Erik, Young, Wendy
Zdroj: JMB Online (Journal of Molecular Biology); April 2001, Vol. 307 Issue: 5 p1451-1486, 36p
Abstrakt: We describe a new serine protease inhibition motif in which binding is mediated by a cluster of very short hydrogen bonds (<2.3 Å) at the active site. This protease-inhibitor binding paradigm is observed at high resolution in a large set of crystal structures of trypsin, thrombin, and urokinase-type plasminogen activator (uPA) bound with a series of small molecule inhibitors (2-(2-phenol)indoles and 2-(2-phenol)benzimidazoles). In each complex there are eight enzyme-inhibitor or enzyme-water-inhibitor hydrogen bonds at the active site, three of which are very short. These short hydrogen bonds connect a triangle of oxygen atoms comprising OSer195γ, a water molecule co-bound in the oxyanion hole (H2Ooxy), and the phenolate oxygen atom of the inhibitor (O6′). Two of the other hydrogen bonds between the inhibitor and active site of the trypsin and uPA complexes become short in the thrombin counterparts, extending the three-centered short hydrogen-bonding array into a tetrahedral array of atoms (three oxygen and one nitrogen) involved in short hydrogen bonds. In the uPA complexes, the extensive hydrogen-bonding interactions at the active site prevent the inhibitor S1 amidine from forming direct hydrogen bonds with Asp189 because the S1 site is deeper in uPA than in trypsin or thrombin.
Databáze: Supplemental Index
Externí odkaz: