| Autor: |
Paton, V G, Shackelford, J E, Krisans, S K |
| Zdroj: |
Journal of Biological Chemistry; July 1997, Vol. 272 Issue: 30 p18945-50, 6p |
| Abstrakt: |
To date, isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase; EC 5.3.3.2) is presumed to have a cytosolic localization. However, we have recently shown that in permeabilized cells lacking cytosolic components, mevalonate can be converted to cholesterol, implying that all of the enzymes required for the conversion of mevalonate to farnesyl diphosphate are found in the peroxisome. To provide unequivocal evidence for the subcellular localization of IPP isomerase, in this study, we have cloned the rat and hamster homologues of IPP isomerase and identified the signal that targets this enzyme to peroxisomes. In addition, we also demonstrate that IPP isomerase is regulated at the mRNA level. |
| Databáze: |
Supplemental Index |
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