| Autor: |
Sawicki, Mark, Arnold, Elizabeth, Ebrahimi, Sam, Duell, Thomas, Jin, Sang, Wood, Thomas, Chakrabarti, Rita, Peters, Julius, Wan, Yvonne, Samara, Ghasan, Weier, Heinz-Ulrich G., Udar, Niton, Passaro, Edward, Srivatsan, Eri S. |
| Zdroj: |
Genomics; June 1997, Vol. 42 Issue: 3 p405-412, 8p |
| Abstrakt: |
A transcription map of a 1200-kb region encompassing theMEN1locus was constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to theMEN1consensus region. Since many of the sequences cloned by cDNA selection also identified ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by PCR amplification of genomic clones. Nine known genes, 2 putative human homologues to mouse genes, and 18 novel transcripts map to the region. Transcripts that map to theMEN1intervalPYGM–D11S449 include SGC35223, IB1256, AA147620,ZFM1, FAU,andCAPN1.The latter 3 known genes have already been excluded as candidateMEN1genes. The 2 putative human homologues of mouse genesLtbp2andSpa-1may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside theMEN1consensus region they may play a role in sporadic endocrine tumors independent of theMEN1gene or in other tumors, such as breast cancer, that have loss of heterozygosity within this region. |
| Databáze: |
Supplemental Index |
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