| Autor: |
Zhulidov, P. A., Bogdanova, E. A., Shcheglov, A. S., Shagina, I. A., Wagner, L. L., Khazpekov, G. L., Kozhemyako, V. V., Lukyanov, S. A., Shagin, D. A. |
| Zdroj: |
Russian Journal of Bioorganic Chemistry; March 2005, Vol. 31 Issue: 2 p170-177, 8p |
| Abstrakt: |
Abstract We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, effectively cleaves double-stranded DNA, and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935–1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink