Targeted Development of Microsatellite Markers from Inter-AluAmplification of YAC Clones

Autor: de Souza, Anete Pereira, Allamand, Valérie, Richard, Isabelle, Brenguier, Lydie, Chumakov, Ilya, Cohen, Daniel, Beckmann, Jacques S.
Zdroj: Genomics; January 1994, Vol. 19 Issue: 2 p391-393, 3p
Abstrakt: Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alusequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
Databáze: Supplemental Index