| Autor: |
Smith, Craig A., Hu, Fang-Qi, Smith, Terri Davis, Richards, Cheryl L., Smolak, Pamela, Goodwin, Raymond G., Pickup, David J. |
| Zdroj: |
Virology; September 1996, Vol. 223 Issue: 1 p132-147, 16p |
| Abstrakt: |
We show the cowpox genome (Brighton Red strain) contains a single copy gene,crmC,expressed at late times during viral infection, encoding a soluble, secreted protein whose sequence marks it as a new member of the TNF receptor family. The cysteine-rich protein contains 186 amino acids, the N-terminal 21 of which constitute a signal peptide, and two potential N-linked glycosylation sites. The ∼25-kDa recombinant protein binds TNF specifically and completely inhibits TNF-mediated cytolysis. The strongest sequence homologues are the ligand-binding regions of the type II cellular TNF receptor (TNFRII) and CrmB, a distinct pox virus gene also encoding a soluble TNF binding protein. Unlike TNFRII and CrmB, CrmC does not bind lymphotoxin (LTα, TNFβ) and lacks the conserved (but nonhomologous) ∼150-residue C-terminal domain of CrmB proteins. The presumed function of CrmC is viral inhibition of host-elicited TNF. |
| Databáze: |
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