| Autor: |
Davidow, Lance S., Barberio, Dana, Cao, Jie, McCormick, Irene |
| Zdroj: |
Molecular and Cellular Probes; June 1994, Vol. 8 Issue: 3 p241-244, 4p |
| Abstrakt: |
The largest of the commonly used probes for Southern blot diagnosis of fragile X mental retardation syndrome spans the CGG repeat cluster in the FMR-1 gene. This probe causes appearance of 'common' or 'constant' background bands which occasionally complicate the interpretation of autoradiographic results. By removing a 357 bp Sphl to Nhel fragment containing the CGGs from the probe pE5·1, we constructed a probe which eliminates the background bands yet allows the use of a large probe (4·8kb) to detect changes in the diagnostic 5·2 kb genomic EcoRI band. This CGG-deficient probe has been used in routine diagnostic cases as well as in second round testing of pE5·1-probed cases where enlarged mutant bands are suspected to comigrate with the background bands. Copyright 1994, 1999 Academic Press |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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