| Autor: |
Goenka, Shradha, Das, Tanya, Sa, Gaurisankar, Ray, Prasanta K. |
| Zdroj: |
Biochemical and Biophysical Research Communications; September 1998, Vol. 250 Issue: 2 p425-429, 5p |
| Abstrakt: |
Protein A ofS. aureusexhibits a wide array of immunopotentiating activities. Since the role of nitric oxide (NO) in bioregulation has been well envisaged, we studied the effect of Protein A on NO production by immunocytes bothin vivoandin vitro.Our data indicate that PA at a comparable dose of LPS (lipopolysaccharide) increases the NO levels in the serum of Swiss albino mice by about 12-fold from its basal level. The peak level is reached at about 12 hours after i.p. inoculation of PA. However, NO concentration returns to the basal value 15 hours posttreatment. Splenic lymphocytes and peritoneal macrophages showed appreciable increase in NO production when cultured with PAin vitro.Interestingly, inhibitors of tyrosine kinase, phospholipase C, and protein kinase C (PKC) inhibited NO production in splenic lymphocytes. Thus, it appears that these enzymes participate in the signaling cascade induced by PA, which culminates in the production of NO downstream of PKC. It is possible that PA-induced NO production may have relevance with the anti-tumor and anti-parasitic properties of PA, described earlier. |
| Databáze: |
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