| Autor: |
Matson, R.S., Rampal, J., Pentoney, S.L., Anderson, P.D., Coassin, P. |
| Zdroj: |
Analytical Biochemistry; January 1995, Vol. 224 Issue: 1 p110-116, 7p |
| Abstrakt: |
High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64-channel fluidic chemical delivery system is used to prepare the 64 × 64 array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment.Copyright 1995, 1999 Academic Press, Inc. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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