| Autor: |
KLOK, ERIK JAN, LUBSEN, NICOLETTE H, CHAMBERLAIN, CORAL G, McAVOY, JOHN W |
| Zdroj: |
Experimental Eye Research; October 1998, Vol. 67 Issue: 4 p425-431, 7p |
| Abstrakt: |
The differentiation of rat lens epithelial cells to fibre cells can be mimicked using lens epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor (FGF). A previous study demonstrated that FGF is required only for initiation of differentiation: once induced by FGF, differentiation can be maintained by insulin (as assessed by following the accumulation of fibre-cell specific crystallins). The aim of this investigation was to determine whether insulin-like growth factor 1 (IGF-1) can also maintain differentiation and to include a cellular analysis of explants undergoing insulin-or IGF-maintained differentiation in vitro. Measurement of the accumulation of α-, β- and γ-crystallins showed that IGF-1, like insulin, can replace FGF-2 in directing the pulses of α-, β- and γ-crystallin gene expression once differentiation is initiated by FGF-2. Cells in both the peripheral and the central region of the explants responded. Immunolocalization of α, β- and γ-crystallins in these explants showed that a 15 min pulse of FGF-2 triggered the differentiation of only a few cells, whereas a 12 hr pulse primed virtually all the cells for differentiation. This indicates that in explants, individual cells differ in the rate at which they can respond to FGF-2. |
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