| Autor: |
YOUSIF, M.H., ORIOWO, M.A., WILLIAMS, K.I. |
| Zdroj: |
Pharmacological Research; November 1998, Vol. 38 Issue: 5 p381-386, 6p |
| Abstrakt: |
Sodium nitroprusside (SNP) (10−9–10−6mol) and carbachol (10−9–10−6mol) induced dose-dependent vasodilatation in the perfused rabbit isolated ovarian vascular bed. Carbachol, but not SNP-induced vasodilatation was abolished by treatment with CHAPS (4.7 mg ml−1, 30 s) to remove the endothelium. Carbachol-induced responses were also significantly attenuated by LY 83583 (10−5M) and methylene blue (3×10−5M).L-NOARG (10−5M) reduced carbachol-induced vasodilatation. None of these compounds affected SNP-induced vasodilator responses. Both SNP- and carbachol-induced vasodilatation were attenuated by raising the [K+] in the Krebs’ solution to 40 mM. The responses were also reduced by TEA (20 mM) but not by glibenclamide. It was therefore concluded that SNP induced cGMP-independent vasodilator responses in the perfused rabbit ovarian vascular bed. This vasodilator response involved membrane hyperpolarisation since it was lost in high [K+] Krebs’ solution. |
| Databáze: |
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