| Autor: |
Bagavandoss, P., Kaytes, P., Vogeli, G., Wells, P.A., Wilks, J.W. |
| Zdroj: |
Biochemical and Biophysical Research Communications; April 30, 1993, Vol. 192 Issue: 2 p325-332, 8p |
| Abstrakt: |
The angiogenic and malignant phenotypes of hamster tumor cells are inversely correlated with the expression of an amino terminally truncated thrombospondin (TSP) subunit. In the present study, we have constructed a truncated TSP subunit from a human fibroblast cDNA library (rt-TSP1) and expressed it in Chinese hamster ovary (CHO) cells. Increased concentrations of plasminogen activator inhibitor-1 (PAI-1) were detected in endothelial cell conditioned medium following treatment with rt-TSP1. This rt-TSP1-induced increase in PAI-1 was neutralized by monoclonal antibodies to both TSP and TGFβ. rt-TSP1 also inhibits the proliferation of endothelial cells and this response is also neutralized by TSP and TGFβ antibodies. Serine and cysteine proteases inhibitors were used to determine if rt-TSP1 activated the latent TGFβ. However, these protease inhibitors did not neutralize the effect of rt-TSP1. The data indicate that the anti-angiogenic properties of TSP may be due to inhibition of the pericellular proteolysis required for endothelial cell migration and endothelial cell proliferation.Copyright 1993, 1999 Academic Press |
| Databáze: |
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