| Autor: |
Spatz, Martin, Ali, Stuart A., Auer, Manfred, Graf, Christine, Eibl, Martha M., Steinkasserer, Alexander |
| Zdroj: |
Protein Expression and Purification; June 1999, Vol. 16 Issue: 1 p40-46, 7p |
| Abstrakt: |
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>5 mg/108cells) of ICP8. The recombinant ICP8 is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% α-helix and 15.4% β-sheet. This is the first structural report for ICP8 using CD analysis, which will be very useful for high-throughput assay development and mechanistic studies. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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