| Autor: |
Davies, Eryl V., Hallett, Maurice B. |
| Zdroj: |
Biochemical and Biophysical Research Communications; July 1998, Vol. 248 Issue: 3 p679-683, 5p |
| Abstrakt: |
Ca2+near the inner face of the plasma membrane, as reported by the membrane associated fluorescent Ca2+probe FFP-18, was higher than the bulk cytosolic free Ca2+concentration both in resting neutrophils and in response to f-met-leu-phe. Influx caused Ca2+close to the plasma membrane to rise more rapidly than the bulk cytosolic free Ca2+and to reach a peak concentration of at least 30 μM. This zone of high Ca2+was localised to just beneath the plasma membrane and did not extend more than 0.1 μm into the cell, as it was undetected by the bulk cytosolic free Ca2+probes magfura2 and fura2. From these data, reconstruction of the distribution of Ca2+within the neutrophil showed that the high Ca2+signal at the cell cortex rapidly subsided to give a uniform free Ca2+across the cell. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect