| Autor: |
Maruoka, Hidenori, Ikarashi, Yoshinori, Shinohara, Kouichi, Miyata, Michio, Sugimura, Takashi, Terada, Masaaki, Wakasugi, Hiro |
| Zdroj: |
Biochemical and Biophysical Research Communications; January 1998, Vol. 242 Issue: 2 p413-418, 6p |
| Abstrakt: |
By immunizing mouse lymphoma cell line tMK-2U derived from intermediate TCR cells of BALB/c nude mouse, U5A2-13 monoclonal antibody (mAb, a rat IgG2a) was established. U5A2-13 antigen (Ag) was expressed on around 65% of TCRintcells in the liver of the various mouse strains including both NK1.1−and NK1.1+mouse strains, while NK1.1 Ag was expressed only in NK1.1+C57BL/6 mouse strain. Among CD3+cells, 26.3% cells co-expressed U5A2-13 Ag and NK1.1+Ag, while small proportions of the CD3+cells were U5A2-13+NK1.1−(9.2%) or U5A2-13−NK1.1+(4.4%). Among NK1.1+cells, 54.9% cells co-expressed CD3 and U5A2-13 Ag, while some proportions of the cells were U5A2-13+CD3−(19.4%) or U5A2-13−CD3+(9.8%). It was found that approximately 85% of NK1.1+CD3+cells co-expressed U5A2-13 Ag. U5A2-13 Ag with low fluorescence intensity was also expressed on 55% of NK1.1+CD3−NK cells. U5A2-13 Ag immunoprecipitated from tMK-2U cells consisted of three proteins, which were 65 kDa, 33 kDa and 32 kDa under both reducing and non-reducing conditions and these were apparently different from NK 1.1 Ag. These results indicated that U5A2-13 mAb was able to define a similar population to NK1.1+CD3+T cells and to 55% of NK1.1+CD3−NK cells in various strains, through recognizing a different molecule from NK1.1 Ag. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect