| Autor: |
Ashraf, Md Musharraf, Murakami, Makoto, Shimbara, Satoko, Amakasu, Yoshihisa, Atsumi, Gen-ichi, Kudo, Ichiro |
| Zdroj: |
Biochemical and Biophysical Research Communications; December 1996, Vol. 229 Issue: 3 p726-732, 7p |
| Abstrakt: |
Treatment of murine bone marrow-derived mast cells (BMMC) with interleukin (IL)-10 and IL-1β increased the expression of type II secretory phospholipase A2(sPLA2), but not cytosolic PLA2(cPLA2), after culture for several hours. Further stimulation with IgE and antigen (IgE/Ag) increased cyclooxygenase (COX)-2 expression dramatically, accompanied by augmented delayed prostaglandin (PG) D2generation over several hours. BMMC were also found to express type IIC PLA2(PLA2-IIC), a recently described novel sPLA2possessing 16 cysteine residues, expression of which changed minimally after BMMC activation. Delayed PGD2generation was suppressed by ∼80% by an antibody raised against recombinant murine type II sPLA2. These results suggest that, of the three PLA2s expressed in BMMC, type II sPLA2is the critical enzyme that is coupled with COX-2-dependent PGD2generation elicited by IgE/Ag in the presence of IL-10 + IL-1β. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect