| Autor: |
Abe, Takako K., Tanaka, Hiroshi, Iwanaga, Toshihiko, Odani, Shoji, Kuwano, Ryozo |
| Zdroj: |
Biochemical and Biophysical Research Communications; April 1997, Vol. 233 Issue: 2 p295-299, 5p |
| Abstrakt: |
Membrane proteins in growth cone-enriched and growth cone-non-enriched fractions prepared from neonatal mouse brain were separated by lectin-affinity and ion exchange chromatographies, 2D-PAGE, and SDS-PAGE. Partial amino acid sequences of the proteins concentrated in the growth cone-enriched fraction were determined. We found that one such protein, gmp23-48k, corresponds to the 50-kDa subunit (p50) of the dynactin complex. An antibody raised against gmp23-48k strongly reacted with growth cones of differentiated neuronal precursor cells. Immunoblot analyses revealed that gmp23-48k was present both in membrane and in soluble fractions of neonatal brain. However, the amount of gmp23-48k in the membrane fraction greatly decreased in adult brain. These results suggest a special role of membrane-associated gmp23-48k/p50 in synapse formation during brain development. |
| Databáze: |
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