| Autor: |
Claros, M.C., Papke, Y., Kleinkauf, N., Adler, D., Citron, D.M., Hunt-Gerardo, S., Montag, Th., Goldstein, E.J.C., Rodloff, A.C. |
| Zdroj: |
Anaerobe; June 1999, Vol. 5 Issue: 3-4 p137-140, 4p |
| Abstrakt: |
Fusobacterium ulceransis a newly described obligately anaerobic Gram-negative, non-spore-forming rod [1] that has been isolated from tropical ulcers. Two morphotypes were described: one resemblingFusobacterium variumand the other Fusobacterium mortiferum[1]. Because of the weak or negative fermentation reactions of most fusobacteria, the standard carbohydrate tests used for identification of anaerobe organisms are of little use for identification, and other rapid and simple methods are needed. We characterized eight F. ulceransstrains using conventional biochemical testing. We further analysed these strains by PCR employing a single non-specific primer AP3 and by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of whole cell proteins. PCR using a self-designed pair of primers for the amplification of the spacer (intergenic) region between the 16S and 23S rRNA genes, led to the development of genetic markers for species identification. All F. ulceransclinical isolates appeared very similar to each other in all the test parameters, but were distinctly different from the type strains of the two phenotypically similar species, F. mortiferumand F. varium. High similarity in PCR- and protein-profiles also raise the possibility that all these F. ulceransstrains came from one clone. We noted significant differences among the strains of F. mortiferumand F. varium. |
| Databáze: |
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