| Autor: |
Lecoq, D, Hervagault, J F, Broun, G, Joly, G, Kernevez, J P, Thomas, D |
| Zdroj: |
Journal of Biological Chemistry; July 1975, Vol. 250 Issue: 14 p5496-5500, 5p |
| Abstrakt: |
Artificial membranes bearing immobilized enzymes can be used to study some effects of membrane structure on enzyme kinetic behavior. The bienzyme system described is a mixture of beta-glucosidase and glucose oxidase. Gluconolactone, the product of thesecond enzyme, is an inhibitor of the first one. The resulting feedback effect has been compared using a mixed two-enzyme membrane, two separated one-enzyme membranes, and astirred bienzyme solution. The feedback effect is quicker and more efficient in the two-enzyme membrane than in solution; it is slower and less efficient in the case of the separated one-enzyme membranes. Effects of enzyme proximity in the structure are discussed. Conclusions are drawn concerning the efficiency of feedback mechanisms when enzymes are embedded within a single structure. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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