| Abstrakt: |
Optical sectioning in fluorescence microscopy is a cornerstone technique that allows scientists to visualize three-dimensional architectures of specimens with high resolution. The optical sectioning capability has become crucial for advancing our understanding in various technical and scientific fields such as cellular and developmental biology. Several microscopy techniques stand out due to their ability to perform true optical sectioning through distinct optical arrangements. The paper explains formally, how optical sectioning is harnessed in confocal, two-photon, confocal theta, and light sheet-based fluorescence microscopy. The latter’s implementation of optical sectioning fosters detailed studies with morphologically intact fertile specimens as a function of long time periods. |
