Cloning and Localization of the MurineXpctGene: Evidence for Complex Rearrangements during the Evolution of the Region around theXistGene

Autor: Debrand, Emmanuel, Heard, Edith, Avner, Philip
Zdroj: Genomics; March 1998, Vol. 48 Issue: 3 p296-303, 8p
Abstrakt: The overall organization of the X-inactivation center (XIC/Xic) candidate region seems poorly conserved between human and mouse. The orientation of a region containing the X-inactive-specific transcript (Xist/XIST) gene and three genes located 3′ ofXist/XISThas been shown to be inverted between the two species, although the actual extent of this rearrangement is unknown. We have cloned and mapped the mouse homolog of the humanXPCT(X-linked PEST-containing transporter) gene, which encodes a putative transmembrane transporter. HumanXPCTis located about 200 kb outside of theXICcandidate region and 600 kb 5′ of or telomeric to theXISTgene. The mouseXpctgene, which lies approximately 300 kb 5′ of and centromeric toXist,displays 85% identity at the nucleotide level with the human gene, and the overall protein structure is conserved. The transcriptional orientation of mouseXpctwith respect toXistis the opposite of that in human. Consequently, the evolution of the region between human and mouse appears to be highly complex, with structural rearrangements involving a region of up to 600 kb or more around theXistgene.
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