| Abstrakt: |
Indiscriminate disposal of chicken feather waste into the environment causes severe pollution, adverse environmental effects, and facilitates emergence of pathogens. The present study has been aimed to isolate keratinolytic bacteria from soil, screen, identify, and optimize culture conditions for them and to check the degradation potential of the isolated bacteria on pre-treated feather. Three bacteria exerted better keratinolytic activity among other strains, and based on 16S rRNA sequencing, they were identified as Pseudomonas aeruginosa, Rosselomorea marisflavi, and Exiguobacterium indicumrespectively. Chicken feathers were pre-treated with various chemicals at different concentrations, in that NaOH pre-treatment solubilized feather in a better way and was used as substrate for the culture of the aforesaid bacteria. Exiguobacterium indicumeffectively degraded 0.1 N NaOH treated feather among other strains and displayed better keratinolytic property at 37 °C and pH 9 respectively. The protein and amino acid present in the E. indicumculture soup was found to be 3.71 mg/ml and 3.61 mg/ml respectively and its proteolytic activity was 62.12 U/ml. Scanning electron microscope (SEM) images of the feather confirmed degradation and Fourier transform infrared (FTIR) analysis revealed the destruction of peptide bonds that testified degradation. In the antibiotic sensitivity assay, all the three bacteria was found to be sensitive to chloramphenicol and streptomycin. It has been concluded that E. indicumis capable of degrading pre-treated feather in an efficient manner and has the potential to transform feather waste to valuable products. |
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