| Autor: |
Hou, Yuluan, Zhang, Ximei, Zeng, Donglan, Zhu, Shangling, Luo, Yang, Dang, Junlong, Wu, Wenbin, Xiong, Yiding, Zhao, Jun, Huang, Jianlin, Yuan, Jia, Wang, Shuhong, Wang, Julie, Xu, Hanshi, Zhang, Wei, Ai, Hong, Chen, Zheng, Zheng, Song Guo |
| Zdroj: |
View; December 2024, Vol. 5 Issue: 6 |
| Abstrakt: |
A therapeutic strategy using mesenchymal stem cells (MSCs) has been accepted as a novel therapy for treating rheumatoid arthritis (RA). Human gingiva‐derived MSCs (GMSCs) are superior in regulating immune responses. Autologous MSCs are the optimal candidate to avoid the potential risks of allogenic MSCs. However, whether autologous GMSCs from RA patients are therapeutic remains unknown. In this study, we compared the therapeutic efficacy of GMSCs derived from patients with RA (RA‐GMSCs) and that from health donors (H‐GMSCs) in vivo and in vitro. Then, we utilized RNA‐sequencing, the molecular and cellular assays to determine the immunomodulatory molecules that contribute to the therapeutic effect of RA‐GMSCs on both collagen‐induced arthritis (CIA) and humanized synovitis models. We demonstrated that GMSCs derived from patients with RA (RA‐GMSCs) and health donors (H‐GMSCs) shared a similar expression of immunomodulatory molecules. Moreover, RA‐GMSCs were as effective as H‐GMSCs in suppressing T‐cell proliferation, proinflammatory cytokines secretion, as well as osteoclast differentiation in vitro. In addition, RA‐GMSCs had a robust therapeutic effect on the CIA model. Importantly, RA GMSCs can survive for at least 24 days in a CIA mouse model and can be distributed in the spleen, lymph nodes, and joints. Specifically, RA‐GMSCs decreased the frequency of Th1 and Th17 cells whereas enhanced Treg cells, reducing the joint histopathological scores of lymphocytes, osteoclasts, and cartilages. Moreover, RA‐GMSCs were also effective in suppressing inflamed synoviocyte proliferation, migration, and invasion in vitro, and cartilage invasion in a humanized synovitis model in vivo. Our study implies that manipulation of RA‐GMSCs is therapeutic in CIA mice and humanized synovitis models and may have therapeutic potential in RA patients using autologous GMSCs in the future. The current study elucidates RA‐GMSCs shared the similar immunophenotypes to H‐GMSCs. RA‐GMSCs can suppress T cell immune responses and osteoclastogenesis in vitro. Importantly, RA‐MGSCs can exert a therapeutic role in treating CIA and humanized synovitis models. The aforementioned data indicate that the administration of autologous GMSCs may have a promising therapeutic role in patients with RA. |
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