Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluorescence in-situhybridization with a subtelomeric probe

Autor: Benedek, Katalin, Chudoba, Ilse, Klein, George, Wiener, Francis, Mai, Sabine
Zdroj: Chromosome Research; December 2004, Vol. 12 Issue: 8 p777-785, 9p
Abstrakt: Previous work has demonstrated that chromosome 11 is trisomic or shows a duplicated region encompassing the E1/E2 band of chromosome 11 in 90% of v-abl/myc-induced plasmacytomas (Wiener et al.1995). In the present report, we have studied BALB/c PreB lymphocytes that were immortalized by v-abland stably transfected with a conditional MycER vector (Mai et al.1999). These cells, termed PreB ABL/MYC, showed changes in the E1/E2 bands of chromosome 11 that are similar to those reported previously for v-abl/myc-induced plasmacytomas. This was shown by the use of chromosome painting, SKY, FISH and mBAND. Our findings suggest that the Pre-B ABL/MYC cells may be used to analyse the genetic changes affecting chromosome 11 that are associated with v-abl/myc-dependent tumorigenesis in mouse B cells.
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