| Autor: |
Chen, Fuhua, Mottino, Giuliano, Shin, Victoria Y., Frank, Joy S. |
| Zdroj: |
Journal of Molecular and Cellular Cardiology; October 1997, Vol. 29 Issue: 10 p2621-2629, 9p |
| Abstrakt: |
Ankyrins are a multigene family of proteins that function as adapters between the cytoskeleton and trans-membrane proteins, such as ion channels. Previous studies have shown the linkage between ankyrin and ionic transport proteins such as Na+–K+ATPase, voltage-dependent Na+channels and Ca2+channels. In the present study, we have investigated the subcellular distribution of ankyrin and its relationship to the Na+–Ca2+exchange protein in immature and adult rabbit ventricular myocytes. Isolated single cardiomyocytes from neonatal, juvenile and adult rabbit hearts were examined by immunofluorescence labeling techniques, using antibodies against ankyrin and the Na+–Ca2+exchanger. We found that in neonatal rabbit cardiac myocytes, ankyrin labeling was mainly present at the Z disk, whereas the Na+–Ca2+exchanger was only present on the peripheral sarcolemma. At 2 weeks of age, ankyrin labeling was still predominantly observed at the level of the Z disks as well as in the partially developed T-tubules. In the adult cells, however, ankyrin and the Na+–Ca2+exchanger seem to be co-localized within T-tubules and at the costamere region of the peripheral sarcolemma. Immunogold labeling studies at the higher resolution electron microscopic level using cyrosection tissues of rabbit heart at different ages confirm these findings. These results indicate that the distribution pattern of ankyrin and the Na+–Ca2+exchanger changes with development in rabbit ventricular myocytes. |
| Databáze: |
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