| Autor: |
Bai, Songjie, Hu, Ruomeng, Wen, Xuehuan, Zhang, Wenting, Li, Bingyu, Yang, Jie, Yu, Qing, Zeng, Xianchang, Xiu, Huiqing, Cai, Zhijian, Zhang, Gensheng |
| Zdroj: |
STAR Protocols; March 2025, Vol. 6 Issue: 1 |
| Abstrakt: |
Extracellular vesicles (EVs) play a crucial role in delivering bioactive cargo in infectious diseases. Here, we present a protocol for isolating EVs from alveolar macrophages (AMs) that phagocytose methicillin-resistant Staphylococcus aureus(MRSA) in vitrocell culture models. We describe steps for bacterial preparation; infection of AMs with MRSA; and isolation, purification, and characterization of EVs. This protocol provides a valuable perspective for studying EVs derived from pathogen-infected immune cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
