| Autor: |
Tachibana, Hirofumi, Kubo, Takao, Miyase, Toshio, Tanino, Sousuke, Yoshimoto, Miki, Sano, Mitsuaki, Yamamoto-Maeda, Mari, Yamada, Koji |
| Zdroj: |
Biochemical and Biophysical Research Communications; January 12, 2001, Vol. 280 Issue: 1 p53-60, 8p |
| Abstrakt: |
IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ɛ germline transcript (ɛGT), which is critical to initiate IgE production. While searching for molecules that inhibit ɛGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced ɛGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced ɛGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced ɛGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells. |
| Databáze: |
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