| Autor: |
Zhang, Zhesheng, Zhao, Jinshan, Zang, Jinhong, Peng, Chuantao, Lv, Liangtao, Li, Zhaojie |
| Zdroj: |
Food Bioscience; December 2024, Vol. 62 Issue: 1 |
| Abstrakt: |
The inhibitory mechanism of postbioYDFF-3 (postbiotics) on Pseudomonas putida NBRC 14164(P. putida NBRC 14164) and Acinetobacter johnsonii ANC 3681(A. johnsonii ANC 3681) in refrigerated fish fillet was investigated. At the cellular level, the minimum inhibitory concentrations of P. putida NBRC 14164and A. johnsonii ANC 3681treated with postbiotics were 3.9063 μL/mL and 15.625 μL/mL, respectively. The postbiotics effectively inhibited biofilm formation, promoted cell DNA leakage and increased cellular membrane permeability. Furthermore, the transcriptomic results showed that the pathways of transmembrane transport, energy metabolism, DNA damage repair, nucleotide binding and oxidation-reduction (REDOX) were significantly down-regulated. In particular, PhnA and A1_GM000335 genes were up-regulated by 9.71 and 7.16 times respectively, which was the key to stress response in the nucleotide binding pathway of A. johnsonii ANC 3681. PP4_RS22590 and katE genes were down-regulated by 28.64 and 11.63 times respectively, which caused the REDOX inhibition of P. putida NBRC 14164. The transcriptomic results were verified by quantitative real-time PCR (qRT-PCR). This study provided a new theoretical basis for the inhibition of postbiotics against P. putida NBRC 14164and A. johnsonii ANC 3681. |
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