| Autor: |
Ernø, Henrik, Küry, Patrick, Nitsch, Cordula, Jost, Jean-Pierre, Monard, Denis |
| Zdroj: |
Molecular and Cellular Neurosciences; July 1996, Vol. 8 Issue: 1 p28-37, 10p |
| Abstrakt: |
The expression of the serine protease inhibitorProtease nexin-1 (PN-1)is upregulated in glial cells following different types of lesion in the nervous system. A strong negative regulatory element has been shown by the missing nucleoside technique to be a CACGTG site (E-box) in the proximal part of thePN-1promoter. The factor binding to this site is specifically recognized by antibodies directed against the human upstream stimulatory factor (USF). Point mutations in the E-box binding site which abolish USF bindingin vitroincrease the transcriptional activity of thePN-1promoter. Cotransfection of aPN-1promoter/reporter construct together with an expression vector for humanUSF1confirms the negative regulatory function of this site. Finally, we show that the binding to this USF site changed after ibotenic acid-induced lesion of the caudate putamen in the rat brain. |
| Databáze: |
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