| Autor: |
Darish, Joseph R., Kaganer, Alyssa W., Hanley, Brenda J., Schuler, Krysten L., Schwabenlander, Marc D., Wolf, Tiffany M., Ahmed, Md Sohel, Rowden, Gage R., Larsen, Peter A., Kobashigawa, Estela, Tewari, Deepanker, Lichtenberg, Stuart, Pedersen, Joel A., Zhang, Shuping, Sreevatsan, Srinand |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; 20240101, Issue: Preprints |
| Abstrakt: |
The rapid geographic spread of chronic wasting disease (CWD) in white-tailed deer (WTD; Odocoileus virginianus) increases the need for the development and validation of new detection tests. Real-time quaking-induced conversion (RT-QuIC) has emerged as a sensitive tool for CWD prion detection, but federal approval in the United States has been challenged by practical constraints on validation and uncertainty surrounding RT-QuIC robustness between laboratories. To evaluate the effect of inter-laboratory variation on CWD prion detection using RT-QuIC, we conducted a multi-institution comparison on a shared anonymized sample set. We hypothesized that RT-QuIC can accurately and reliably detect the prions that cause CWD in postmortem samples from medial retropharyngeal lymph node (RPLN) tissue despite variation in laboratory protocols. Laboratories from 6 U.S. states (Michigan, Minnesota, Missouri, New York, Pennsylvania, Wisconsin) were enlisted to compare the use of RT-QuIC in determining CWD prion status (positive or negative) among 50 anonymized RPLNs of known prion status. Our sample set included animals of 3 codon 96 WTD genotypes known to affect CWD progression and detection (G96G, G96S, S96S). All 6 laboratories successfully identified the true disease status consistently for all 3 tested codon 96 genotypes. Our results indicate that RT-QuIC is a suitable test for the detection of CWD prions in RPLN tissues in several genotypes of WTD. |
| Databáze: |
Supplemental Index |
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