| Autor: |
Tinh, Nguyen H., Hop, Nguyen V., Phuong, Pham T., Tam, Trinh L. H., Quoc, Nguyen B., Son, Trinh H., Bui, Anh P. N. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; November 2024, Vol. 36 Issue: 6 p847-851, 5p |
| Abstrakt: |
Piglet lethality is one of the major concerns in pig breeding programs. Deletion of a 212-kb region within the Bardet-Biedl syndrome 9 (BBS9) gene has been linked to a reduction in the number of piglets born alive per litter. The BBS9mutant gene carrier-by-carrier mating scheme could result in mummification of piglets carrying 2 copies of the BBS9mutant allele, which ultimately affects the reproductive performance of the sow. Our aim was to develop a simple, rapid, and cost-efficient method that could be applied in a BBS9mutant gene carrier screening program in low- and middle-income countries within basic laboratory settings. Here, we report an optimized multiplex PCR assay that we have established successfully for detection of a 212-kb deletion within the BBS9genomic sequence. We genotyped 420 animals from Yorkshire, Duroc, and Landrace purebred populations in Vietnam. We found that while the BBS9mutant allele was not identified in Duroc pigs, the frequency of BBS9carriers was 10% in both Yorkshire and Landrace populations. We subsequently validated our results using Sanger sequencing. Our multiplex PCR method could be utilized as a BBS9screening test in pig breeding programs. |
| Databáze: |
Supplemental Index |
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