| Autor: |
Johnson, Dana M., Weeks, Emma N. I., LoVullo, Eric D., Geden, Christopher J. |
| Zdroj: |
Florida Entomologist; October 2024, Vol. 107 Issue: 1 |
| Abstrakt: |
Biological control of house flies, Musca domesticaL. (Diptera: Muscidae) has the potential to improve the efficacy and sustainability of integrated pest management. In a previous study, we demonstrated that three bacteria species (Serratia marcescensBizio [Enterobacteriales: Enterobacteriaceae], Photorhabdus temperataFischer-Le Saux et al. [Enterobacteriales: Enterobacteriaceae], and Pseudomonas protegensRamette et al. [Pseudomonadales: Pseudomonadaceae]) are extremely virulent, inducing rapid morbidity and high mortality in adult house flies when injected into the hemocoel but show little effect when applied topically. Here we tested the hypothesis that topical application of these species in combination with the entompathogenic fungus Beauveria bassiana(Bals. Criv.) Vuill. (Hypocreales: Cordycipitaceae) would result in an increased and rapid mortality if fungal penetration of the fly cuticle allowed for increased delivery of the bacteria into the fly hemocoel. Bacteria and B. bassianawere tested either by application at the same time or by sequential application of the two pathogens 48 h apart. Results indicated little evidence for synergy between B. bassianaand any of the bacterial pathogens. However, P. protegenscaused early mortality (<50 % at 3 days) when applied alone and in combination with B. bassiana. A modified disc diffusion assay indicated that P. temperataand P. protegenshad inhibitory effects on the vegetative growth of B. bassianasuggesting the release of antifungal molecules by the bacteria. Results with P. protegenswere sufficiently encouraging to warrant further investigation of this species and its associated toxins as house fly biological control tools. |
| Databáze: |
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