| Autor: |
Neises, Laura, Delbrouck, Catherine, Schuster, Anne, Rezaipour, Mahsa, Eiden, Kim, Oudin, Anais, Fabian, Carina, Niclou, Simone P., Golebiewska, Anna, Meiser, Johannes |
| Zdroj: |
STAR Protocols; December 2024, Vol. 5 Issue: 4 |
| Abstrakt: |
Herein, we present an ex vivoapproach to study glioblastoma (GBM) cell motility in viable mouse brain slice cultures, closely mimicking in vivofeatures. We detail the preparation and culturing of mouse brain slices followed by tumor cell injection, allowing for the analysis of different aspects of the cellular migration and invasion process. Our assay facilitates testing diverse perturbations including genetic modifications and treatments in a physiological context. Thus, the protocol provides a compromise between in vitroassays and in vivomodels. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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