Similarities in Restriction Fragment Patterns of Mitochondrial DNAs ofPhytophthoraSpecies Strongly Depend on the Restriction Enzyme Used Due to Heterogeneous Base Distribution and Sequence Conservation

Autor: de Cock, Arthur W.A.M., Karlovsky, Petr, Jahnke, Klaus-Dieter, Bahnweg, Günther
Zdroj: Fungal Genetics and Biology; March 1996, Vol. 20 Issue: 1 p36-42, 7p
Abstrakt: Mitochondrial DNAs of six morphologically differentPhytophthoraspecies were digested with 15 restriction enzymes. The numbers of restriction fragments obtained differed considerably from those theoretically expected for random base distribution. Enzymes with relatively many G and C in their recognition sequences produced significantly larger numbers of fragments. Moreover, fragments generated by most of these enzymes were more often shared by two or more species than those from enzymes with more A and T in their recognition sequence. It is concluded that base distribution in mitochondrial DNA ofPhytophthorais heterogeneous,AT-rich stretches occurring scattered over the mitochondrial genome and GC-rich regions present in conserved sequences, presumably genes. A practical consequence for taxonomic RFLP studies is that optimal enzymes can be selected, depending on the desired level of resolution.
Databáze: Supplemental Index
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