| Autor: |
Maroney, P. A., Hannon, G. J., Denker, J. A., Nilsen, T. W. |
| Zdroj: |
The EMBO Journal; November 1990, Vol. 9 Issue: 11 p3667-3673, 7p |
| Abstrakt: |
The trans‐spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans‐splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans‐splicing. The effect on trans‐splicing is not due to undermethylation of the cap structure. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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