| Autor: |
Kendall, G., Wilderspin, A. F., Ashall, F., Miles, M. A., Kelly, J. M. |
| Zdroj: |
The EMBO Journal; September 1990, Vol. 9 Issue: 9 p2751-2758, 8p |
| Abstrakt: |
The genes which encode glycosomal glyceraldehyde‐phosphate dehydrogenase (gGAPDH) of Trypanosoma cruzi are arranged as a tandemly repeated pair on a single chromosome and are identical at the level of nucleotide sequence. They are separated by an intergenic region which contains a 317 base pair sequence with the properties of a retroposon. The genes express a 1.5 kb mRNA and a 38 kd protein. The amino acid sequence contains features characteristic of glycosomal enzymes such as peptide insertions and a C‐terminal extension. However, T. cruzi gGAPDH lacks one of the positively charged ‘hotspot’ motifs which have been proposed as topogenic signals for import into the glycosome, a unique microbody‐like organelle. Molecular modelling of the T. cruzi and T. brucei enzymes suggests that neither structure would fulfil the requirements of the ‘hotspot’ glycosomal import model. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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