| Autor: |
Chou, H.S., Behlke, M.A., Godambe, S.A., Russell, J.H., Brooks, C.G., Loh, D.Y. |
| Zdroj: |
The EMBO Journal; September 1986, Vol. 5 Issue: 9 p2149-2155, 7p |
| Abstrakt: |
Both cDNA and genomic clones of the T cell receptor (TCR) alpha‐ and beta‐chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non‐functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six‐member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7‐14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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